Microphysiological System with Continuous Analysis of Albumin for Hepatotoxicity Modeling and Drug Screening

Microphysiological System with Continuous Analysis of Albumin for Hepatotoxicity Modeling and Drug Screening

Liver plays a crucial role in maintaining homeostasis and human serum albumin (HSA) is one of the most important biomarkers indicating normal liver function. A microfluidic albumin
immunosensor was developed and integrated with the liver-on-a-chip microphysiological system (MPS) for continuous feedback over disease modeling and treatment. A gold-electrode based
electrochemical immunosensor was established by anti-HSA antibody immobilization. The liver MPS was found to be efficient for 6 days long live monitoring of disease modelling and drug treatment. The system emulated and analyzed the real-time toxicity modeling with HSA sensing. The detection limit of integrated sensor was 1µg/ml with successive reproducibility. The proposed
sensor was also validated with metabolic biomarkers’ assays. Molecular assays supported the sensor monitoring and depicted the liver injury and recovery. The liver MPS with integrated
albumin sensor may be a promising platform to mimic real-time drug assessment.

Journal: Journal of Industrial and Engineering Chemistry

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